Abstract Chronic alcohol consumption affects various neurotransmitters, especially those implicated in the transitioning to alcohol use disorders (particularly dopaminergic and CRFergic systems).Few studies have investigated moderate alcohol consumption and its harmful consequences.The objective of this work was to analyze behavioral and neurochemi
Abstract A pressing challenge for coming decades is sustainable and just management of large-scale common-pool resources including the atmosphere, biodiversity and public services.This poses a difficult collective action problem because such resources may not show signs that usage restraint is needed until tragedy is almost inevitable.To solve this
Summary: The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest roneverhart.com by proximity-dependent biotinylation.Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the content of autophagosomes and potentially other sho
A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene.Peripheral blood mononuclear cells (PBMCs) were 2006 nissan altima radio reprogrammed using non-integrating delivery of KFL4, amina muaddi scarpe trasparenti OCT4, SOX2, BCL-XL and c-MYC.The i